- Item # 183320
GMI sells re-certified calibrated Vi-CELL Cell Viability Analyzers. The Vi-CELL provides an automatic and cost effective means to perform the trypan blue dye exclusion method for cell viability analysis. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer. Compared to a manual hemacytometer, the Beckman ViCell automates a formerly laborious process with extremely high accuracy and reliability.
The Vi-CELL allows users to load up to 10 samples at once for easy and automated cell counting. A cell sample is loaded into the system (minimum 600uL, but can be diluted and dilution factored into final result), and the instrument aspirates the sample, mixes in the trypan blue, loads the sample into the chamber and records: cell count/mL(equivalent of 100 “grids”), percent viability, and size distribution. It then performs a clean cycle and repeats for the next sample in the carousel. Sample processing time is approximately 2.5 minutes per sample, although there are several user programmable features that can reduce or increase this time.
The automated trypan blue staining procedure can be customized to ensure consistent and accurate cell counts for simple systems such as CHO cells and more complex samples such as yeast. The robust design provides many years of duty, and can be operated by individuals with a wide variety of skill levels. The high quality, pre-mixed reagents help eliminate variability.
Beckman Coulter Vi-CELL XR Automated Cell Viability Analyzer Specifications
Analysis Time < 2.5 min Capacity Single or 12 position via autoloader Concentration Range 5 x 10^4 to 1 x 10^7 cells/mL Counting Accuracy ± 6% Depth 41 cm (16 in) Digitizing Resolution 1394 x 1040 Height 44.5 cm (17.5 in) Imaging Technology Auto-focus CCD array, Firewire camera Instrument Type Video image through a quartz flow cell Operating System Windows® 98, 2000, XP Overall Analysis Range Size Range: 2 µm – 70 µm Power Requirements 100V, 120V, 220V, or 240V 50/60 Hz, 50 W (65 W max.) Sample Volume 0.5 mL Temperature 10°C to 40°C (50°F to 104°F) Viability Range 0 to 100% Weight 11.3 kg (25 lb) Width 38 cm (15 in)
The cell type function within the Vi-CELL Software enables users to optimize the unit for many different cell species, including CHO Cells, yeast cells, white blood cells, and a host of other common cells. The cell type function takes into account cell clumping, cell circularity, cell brightness, cell diameter, and several other pertinent parameters. This capability enables rapid, accurate, and reliable automated cell counting. Further, the cell type function can be customized for unusual cell species that may be of interest in specific industrial or academic settings.
The Vi-CELL XR offers additional benefits over earlier ViCell models:
- Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
- Increased sample throughput with greater efficiency.
- Reduced sample volume results in less reagent use.
- Auto focus capability increases instrument ease of use.
- Variable aspiration and mixing cycles optimize results.
- Enhanced image magnification provides unparalleled cellular detail.
- Circularity measurement helps isolate debris from cells.